Gold nanoparticles as SERS-substrates for MTT assay

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Several variants of gold nanoparticles were proposed for the detection of formazan formed because of enzymatic reduction of the MTT reagent by E. coli enzymes. It is shown that gold nanostars coated with a micellar stabilizer are the most promising SERS-substrate for the detection of formazan in biological mixtures, reducing the required titer of bacteria by at least an order of magnitude.

作者简介

V. Mushenkov

Lomonosov Moscow State University

Email: vladimir.mushenkov@mail.ru
Moscow, Russia

A. Burov

Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences

Saratov, Russia

V. Kukushkin

Osipyan Institute of Solid-State Physics of the Russian Academy of Sciences

Chernogolovka, Russia

E. Zavyalova

Lomonosov Moscow State University

Moscow, Russia

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