STUDY OF MONONUCLEAR AND TUMOR CELLS MIGRATION TOWRDS TLRS LIGANDS AND CXCL12

Chemotaxis and other factors of innate immunity are involved in metastasis (such as TLRs), it is advisable to study the effect of both TLRs and chemokines on the chemotaxis of tumor cells. The aim. Study and compare the chemotaxis of mononuclear cells and tumor cells towards TLRs ligands and CXCL12. Materials and methods. Chemoattractants were synthetic TLR ligands (DNA_lig, RNA_lig) and CXCL12. The study of cell migration was carried out in dynamics with the help of the Boyden chamber. Results. ^emotaxis of MNC from healthy donors exceeds the chemotaxis of tumor cells by 4 times and significantly differs by 1,6 times from spontaneous migration. Directional chemotaxis of MNCs of healthy donors in 1,7 and 1,5 times greater than the migration of MNCs of healthy donors after 60 minutes and 24 hours, respectively, relative to migration to DNA_lig and RNA_lig. Conclusion. From the results obtained, it can be seen that CXCL12-induced chemotaxis is higher in MNCs of healthy donors than in tumor cells. Similarly, chemotaxis relative to DNA_lig and RNA_lig is significantly lower than in the control. Thus, it can be said that TLRs ligands may inhibit migratory activity.

хемотаксис, TLRs, CXCL12, K562, миеломонобластный лейкоз, Chemotaxis, TLRs, CXCL12, K562, myelomonoblastic leukemia