Method for forming a static biofilm on various surfaces to evaluate the effectiveness of disinfectants

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Abstract

Introduction. In most cases, microorganisms are presented on abiotic surfaces in a state of static biofilm, which determines their resistance to environmental influences, as well as disinfectants. Disinfection regimes for facilities developed by traditional conventional methods using planktonic forms of microorganisms do not provide the required level of disinfection under these conditions. Therefore, it is important to create and validate methods for the formation of static biofilms on surfaces and to study the possibility of their use to assess the effectiveness of disinfectants in the development of disinfection regimes for surfaces in rooms, furniture, appliances, sanitary equipment, etc.

Materials and methods. Microbiological methods, cultural and microscopic were used in the work. The developed new method for evaluating the effectiveness of disinfectants differs in that the microorganisms on the surface of the test objects were in a state of static biofilm. To form it, a suspension of the test microorganism was applied to the test surface, prepared in a GRM broth and incubated in a thermostat at 37°C for 24 hours. The presence of biofilm on the test surface is confirmed by microscopic method. After that, the effectiveness of disinfectants was evaluated.

Results. Using the new method, insufficient activity of disinfectants was shown in concentrations recommended for use by the instructions: tablets based on sodium salt of SDIC at a concentration of 0.03–0.06% for active chlorine, HP at a concentration of 3.0–6.0% and ADBAС at a concentration of 0.25–0.5%. When treating test objects with disinfectant solutions at these concentrations, disinfection was below the 99.99% criterion. To achieve the desired effect, the concentration of working solutions of disinfectants had to be increased for the sodium salt of SDIC to 0.1% for active chlorine, HP to 8.0%, and ADBAС to 1.0%.

Conclusion. The proposed method of forming a static biofilm on abiotic surfaces is a tool for evaluating the effectiveness and developing adequate disinfection regimes for various types of surfaces for practical use.

About the authors

Lyudmila S. Fedorova

Scientific Research Institute for Systems Biology and Medicine

Author for correspondence.
Email: fedorova-ls@yandex.ru
ORCID iD: 0000-0002-3345-2631

D. Sci. (Med.), Professor, Head, Laboratory of overcoming microbial resistance

Russian Federation, Moscow

Anastasia V. Ilyakova

F.F. Erisman Federal Scientific Center of Hygiene

Email: ilyakova.av@fncg.ru
ORCID iD: 0000-0002-1867-3495

researcher, Department of disinfection and sterilization, Institute of disinfectology, Institute of Disinfectology

Russian Federation, Moscow

Alina S. Ivkina

Scientific Research Institute for Systems Biology and Medicine

Email: alinaIvkina21@mail.ru
ORCID iD: 0009-0005-7886-6159

junior researcher, Laboratory of overcoming microbial resistance

Russian Federation, Moscow

Stepan P. Dudik

Scientific Research Institute for Systems Biology and Medicine

Email: stepan_maestro@mail.ru
ORCID iD: 0000-0002-3157-5902

junior researcher, Micro- and nanofluidics laboratory

Russian Federation, Moscow

Elena N. Ilina

Scientific Research Institute for Systems Biology and Medicine

Email: ilinaen@gmail.com
ORCID iD: 0000-0003-0130-5079

D. Sci. (Biol.), Professor, Corresponding Member of the Russian Academy of Sciences, Deputy director for research

Russian Federation, Moscow

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