INDICATORS OF INNATE AND ADAPTIVE IMMUNITY IN EXPERIMENTAL THERMAL INJURY IN THE APPLICATION OF TRANSDERMAL FILMS WITH ERYTHROPOIETIN

The aim of this work is to investigate the effect of transdermal film (TDF) with erythropoietin (EPO) on the parameters of innate and adaptive immunity in experimental thermal injury (TI). The experiment was carried out on 70 white nonlinear Mature rats, which were divided into 3 groups: 1 (n=10) - intact control, 2 (n=30) -TI, 3 (n=30) -TI with the imposition of TDF with EPO. TI IIIA degree with an area of 3.5 % was modeling by immersion of the site interscapular region of the animal in clean water with a temperature of 98-99°С for 12 s. the Study was performed at 3rd, 8th and 14th days TI. The number of neutrophils in the blood was determined by hematological analyzer “Medonic - M 16” (Sweden). The absorption ability of neutrophils blood was examined using of latex particles, the oxygen-dependent metabolism in spontaneous and induced NST-test. With the help of a flow cytometer “Navios” in the blood determined the number of CD3+ and CD45RA+ lymphocytes. The death of lymphocytes was assessed by staining cells with fluorochrome conjugated with annexin V (Annexin-5-FITC) and 7-aminoantipyrine D (7-AAD). It was found that experimental TI in the blood increases the number of neutrophils, their phagocytic and NST-reducing ability, reduced number of CD3+ and CD45RA+ lymphocytes at 5th, 8th and 14th days, increases in blood lymphocyte count with evidence of apoptosis and/or necrosis at 3rd and 8th days. The use of EPO in the composition of TPF in the TI did not accompanied by a change in the number of neutrophils in the blood at 3rd, 8th and 14th days, leads to a decrease in phagocytosis activity at day 14th, the intensity of phagocytosis - on the 8th day, activity and the intensity of spontaneous NST-test at day 14th, the induced NST-test - 3rd, 8th and 14th days; increase of CD3+ lymphocytes in the blood on the 8th day, the reduction in the number of blood lymphocytes with signs of apoptosis and/or necrosis at 14th day.

термическая травма, трансдермальная пленка, эритропоэтин, иммунитет, thermal injury, transdermal film, erythropoietin, immune